Coding
Part:BBa_K1061003:Design
Designed by: mengyi sun Group: iGEM13_SYSU-China (2013-09-24)
RIP 3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 623
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 267
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1198
Design Notes
Although RIP 3 can also induce apoptosis, it mainly induces necrosis via interaction with RIP 1. So the mammalian circuit that aid to use in clinical should use tightly control expression device.
Source
We clone this gene by RT-PCR.