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Coding

Part:BBa_K1061003:Design

Designed by: mengyi sun   Group: iGEM13_SYSU-China   (2013-09-24)


RIP 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1198


Design Notes

Although RIP 3 can also induce apoptosis, it mainly induces necrosis via interaction with RIP 1. So the mammalian circuit that aid to use in clinical should use tightly control expression device.


Source

We clone this gene by RT-PCR.

References